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Immunology and Infection

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Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Article DOI: 10.3791/54554-v • 10:10 min • November 16th, 2016
November 16th, 2016 •
*1, *1, *1, 1, 1,2,3
1Department of Immunology, University of Toronto, 2Toronto General Research Institute, University Health Network, 3Women's College Research Institute
* These authors contributed equally

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This protocol describes how to inoculate C57BL/6J mice with the EGD strain of Listeria monocytogenes (L. monocytogenes) and to measure interferon-γ (IFN-γ) responses by natural killer (NK) cells, natural killer T (NKT) cells, and adaptive T lymphocytes post-infection. This protocol also describes how to conduct survival studies in mice after infection with a modified LD50 dose of the pathogen.

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Listeria Monocytogenes Experimental Infection Host Interferon-gamma Responses Bacterial Load Survival Response To Infection EGG Strain Laboratory Technique Cultivating Bacteria Jennifer Ahn Graduate Student Glycerol Stock Brain Heart Infusion (BHI) Plate Primary Streak Secondary Streak Tertiary Streak Overnight Incubation Uniform Colonies
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