Applications de pHluorin pour Quantitative, Kinetic et à haut débit Analyse des endocytose dans la levure bourgeonnante
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October 23, 2016
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Accurate quantification of vesicular trafficking events often provides key insights into roles for specific proteins and the effects of mutations. This paper presents methods for using superecliptic pHluorin, a pH-sensitive GFP variant, as a tool for quantification of endocytic events in living cells using quantitative fluorescence microscopy and flow cytometry.
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Prosser, D. C., Wrasman, K., Woodard, T. K., O’Donnell, A. F., Wendland, B. Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast. J. Vis. Exp. (116), e54587, doi:10.3791/54587 (2016).
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