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Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)

Article DOI: 10.3791/55042-v • 09:05 min • January 22nd, 2017
January 22nd, 2017 •
1,2, 3, 4, 5,6, 7, 7, 8, 8, 2, 3, 1
1Elemental Bio-imaging Facility, University of Technology Sydney, 2Florey Institute of Neuroscience and Mental Health, The University of Melbourne, 3Department of Pathology, The University of Melbourne, 4School of Earth Sciences, The University of Melbourne, 5Research School, Ruhr University, 6Department of Physiology, Monash University, 7ESI Ltd., Bozeman, 8Agilent Technologies, Mulgrave

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Quantitatively mapping metals in tissue by laser ablation - inductively coupled plasma - mass spectrometry (LA-ICP-MS) is a sensitive analytical technique that can provide new insight into how metals participate in normal function and disease processes. Here, we describe a protocol for quantitatively imaging metals in thin sections of mouse neurological tissue.

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Laser Ablation Inductively Coupled Plasma Mass Spectrometry LA-ICP-MS Metal Imaging Brain Tissue Spatial Distribution Metal Biochemistry Disease Research Neurodegenerative Diseases Therapy Diagnosis Experiment Setup Data Analysis Matrix Matched Tissue Standards Carrier Gas Valve Flow Rate
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