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Shunt Surgery, Right Heart Catheterization, and Vascular Morphometry in a Rat Model for Flow-induced Pulmonary Arterial Hypertension

Article DOI: 10.3791/55065-v • 09:23 min • February 11th, 2017
February 11th, 2017 •
1, 2, 2, 1, 1, 1, 1
1Center for Congenital Heart Diseases, Department of Pediatric Cardiology, Beatrix Children's Hospital, University Medical Center Groningen, University of Groningen, 2Research and Development Facility, University Medical Center Groningen, University of Groningen

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This protocol describes a surgical procedure to create a model for flow-induced pulmonary arterial hypertension (PAH) in rats and the procedures to analyze the principle hemodynamic and histological end-points in this model.

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Shunt Surgery Right Heart Catheterization Vascular Morphometry Flow-induced Pulmonary Arterial Hypertension Rat Model Hemodynamic Endpoints Histological Endpoints Right Heart Catheterization Vascular Morphometry Rat Model Creation Flow-associated Pulmonary Hypertension Intimal-type Vascular Remodeling Pulmonary Blood Flow Standardized Methods Hemodynamic Evaluation Histopathology Characterization Quantitative Morphometry Treatment Strategies Pulmonary Hemodynamics Vascular Morphology Surgery Procedure
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