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Immunology and Infection

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JoVE Journal Immunology and Infection
Human Lung Dendritic Cells
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Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry

Article DOI: 10.3791/55222-v • 11:02 min • January 20th, 2017
January 20th, 2017 •
1, 2, 1, 2, 3, 2, 1
1Immunology and Allergy Unit, Department of Medicine Solna, Karolinska Institutet, 2Division of Medicine, Department of Public Health and Clinical Medicine, Umeå University, 3Divison of Infectious Diseases, Department of Clinical Microbiology, Umeå University

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résumé
Automatic Translation

Lung-resident immune cells, including dendritic cells (DCs) in humans, are critical for defense against inhaled pathogens and allergens. However, due to the scarcity of human lung tissue, studies are limited. This work presents protocols to process human mucosal endobronchial biopsies for studying lung DCs using immunohistochemistry and flow cytometry.

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Human Lung Dendritic Cells Spatial Distribution Phenotypic Identification Endobronchial Biopsies Immunohistochemistry Flow Cytometry Bronchoscopy Mucosal Immunology COPD Sarcoidosis Lung Cancer Enzymatic Digestion Faezzah Baharom Greg Rankin Ume External Environment Pathogens Allergens Toxins Tolerance Immune Response
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