Isolement des mitochondries Intact du muscle squelettique par centrifugation différentielle pour respirométriques mesures haute résolution
18,034 Views
•
10:25 min
•
March 08, 2017
DOI:
Transcription
Automatically generated
Summary
Automatically generated
Here, a quadriceps muscle specimen is taken from an anaesthetized pig and mitochondria are isolated by differential centrifugation. Then, the respiratory rates of mitochondrial respiratory chain complexes I, II and IV are determined using high-resolution respirometry.
Vidéos Connexes
09:01
Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria
Vidéos Connexes
2553 Views
11:27
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
Vidéos Connexes
19449 Views
14:36
Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
Vidéos Connexes
28625 Views
09:45
Mitochondrial Isolation from Skeletal Muscle
Vidéos Connexes
28862 Views
07:03
Robust Mitochondrial Isolation from Rodent Cardiac Tissue
Vidéos Connexes
606 Views
10:35
Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration
Vidéos Connexes
38028 Views
10:12
Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements
Vidéos Connexes
12732 Views
08:54
Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field
Vidéos Connexes
1380 Views
10:25
Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements
Vidéos Connexes
18.0K Views
08:54
Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field
Vidéos Connexes
1.4K Views
Read Article
Cite this Article
Djafarzadeh, S., Jakob, S. M. Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements. J. Vis. Exp. (121), e55251, doi:10.3791/55251 (2017).
Copy