Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Abhijeet Patra; Tao Ding; Minghui Hong; Arthur Mark Richards; Ten It Wong; Xiaodong Zhou; Chester Lee Drum

doi:10.3791/55597

À l’aide de Transmission optique extraordinaire pour quantifier les biomarqueurs cardiaques dans ...

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Bioengineering

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JoVE Journal Bioengineering

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

À l’aide de Transmission optique extraordinaire pour quantifier les biomarqueurs cardiaques dans le sérum humain

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09:23 min

December 13, 2017

DOI: 10.3791/55597-v

Abhijeet Patra¹, Tao Ding², Minghui Hong³, Arthur Mark Richards², Ten It Wong⁴, Xiaodong Zhou⁴, Chester Lee Drum²

¹NUS Nanoscience and Nanotechnology Initiative,National University of Singapore, ²Cardiovascular Research Institute, Yong Loo Lin School of Medicine,National University of Singapore, ³Department of Electrical and Computer Engineering,National University of Singapore, ⁴Institute of Materials Research Engineering,A*STAR (Agency for Science, Technology and Research)

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Overview

This work describes a nanoimprinting lithography method to fabricate high-quality sensing arrays that operate on the principle of extraordinary optical transmission. The biosensor is designed for point-of-care applications, enabling the detection of cardiac troponin I in serum at clinically relevant concentrations.

Key Study Components

Area of Science

Neuroscience
Biotechnology
Biomedical Engineering

Background

The need for reliable biomarker detection in complex biological fluids.
Challenges associated with traditional biosensor technologies.
The principle of extraordinary optical transmission in biosensing.
Importance of point-of-care diagnostics in clinical settings.

Purpose of Study

To develop a low-cost, robust biosensor for cardiac troponin I detection.
To simplify the detection process without complex setups.
To demonstrate the feasibility of extraordinary transmission-based biosensors in clinical applications.

Methods Used

Nanoimprinting lithography for sensor fabrication.
Testing the biosensor in serum samples.
Animation representation of the fabrication process.
Evaluation of detection limits and performance metrics.

Main Results

The biosensor can detect cardiac troponin I at concentrations of 10-400 pg/mL.
It operates effectively in a point-of-care setting.
Demonstrated reliability in detecting biomarkers in complex fluids.
Eliminates the need for elaborate optical or electrical setups.

Conclusions

This method represents a significant advancement in biosensor technology.
It has the potential to improve point-of-care diagnostics for cardiac conditions.
Future work may focus on expanding the range of detectable biomarkers.

Frequently Asked Questions

What is the main application of the biosensor?

The biosensor is primarily used for detecting cardiac troponin I in serum samples.

How does the biosensor work?

It operates on the principle of extraordinary optical transmission, allowing for sensitive detection of biomarkers.

What are the advantages of this biosensor?

It is low-cost, robust, easy to use, and does not require complex setups.

What concentrations can the biosensor detect?

It can detect cardiac troponin I at clinically relevant concentrations ranging from 10 to 400 pg/mL.

Is the sensor fabrication process complex?

The fabrication is performed in a clean room, but the process is simplified for demonstration purposes.

Can this method be used for other biomarkers?

While this study focuses on cardiac troponin I, the method may be adapted for other biomarkers in future research.

Cet ouvrage décrit une méthode de lithographie de dispositifs pour fabriquer des baies de télédétection de haute qualité qui fonctionnent sur le principe de transmission optique extraordinaire. Le biocapteur est peu coûteux, robustes, faciles à utiliser et peut détecter de troponine cardiaque I en sérum aux concentrations cliniquement pertinentes (99^ème percentile coupure ∼10-400 pg/mL, selon le test).

L’objectif global de cette technique est de mesurer les biomarqueurs présents dans un fond de fluide biologique complexe à des concentrations cliniquement pertinentes dans un lieu de soins, sans avoir besoin de configurations optiques ou électriques élaborées. Cette méthode peut aider à faire des biocapteurs basés sur une transmission extraordinaire une réalité dans des scénarios de point of care. Le principal avantage de cette technique est qu’elle ne nécessite pas de configurations électriques ou optiques compliquées.

Dans nos expériences, il a pu détecter de manière fiable des biomarqueurs à des concentrations cliniquement pertinentes. La fabrication de la puce du capteur s’effectue en salle blanche et ne peut pas être filmée. À des fins de démonstration, la procédure est représentée ici par une animation.

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