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Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay

Article DOI: 10.3791/55831-v • 09:11 min • October 2nd, 2017
October 2nd, 2017 •
*1, *1, 2,3, 1,4
1Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education, Shanghai Jiao Tong University School of Medicine, 2Department of Molecular Genetics and Microbiology, Duke University Medical Center, 3Department of Neurobiology, Duke Institute for Brain Sciences, Duke University Medical Center, 4Institute of Health Science, Chinese Academy of Science/Shanghai Jiao Tong University School of Medicine
* These authors contributed equally

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résumé
Automatic Translation

Characterizing the function of odorant receptors serves an indispensable part in the deorphanization process. We describe a method to measure the activation of odorant receptors in real time using a cAMP assay.

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Live-cell Measurement Odorant Receptor Activation Real-time CAMP Assay High-throughput Screening Odorant Screening Mice Odorant Receptor Functioning Pharmacological Cartelizations Odorant-to-receptor Activation Heterologous Expression System Live Cells Phase-contrast Microscope Cell Viability Confluence Estimation Trypsin-EDTA MEM With 10%FBS Cell Count Transfected Cells 96-well Plate Preparation
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