Imaging Neural Activity in the Primary Somatosensory Cortex Using <em>Thy1</em>-GCaMP6s Transgenic Mice

Xiaojing Lin; Tingbao Zhao; Wenhui Xiong; Shaonan Wen; Xiaoming Jin; Xiao-Ming Xu

doi:10.3791/56297

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Imagerie de l’activité neuronale dans le Cortex somatosensoriel primaire utilisant Thy1-GCaMP6s des souris transgéniques

Imaging Neural Activity in the Primary Somatosensory Cortex Using <em>Thy1</em>-GCaMP6s Transgenic Mice

Journal JoVE
Neurosciences

This content is Free Access.

Journal JoVE Neurosciences

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Imagerie de l’activité neuronale dans le Cortex somatosensoriel primaire utilisant Thy1-GCaMP6s des souris transgéniques

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11,039 Views

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07:04 min

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January 07, 2019

DOI:

10.3791/56297-v

DOI:

10.3791/56297-v

•

07:04 min

January 07, 2019

•

10982 Views

Xiaojing Lin^1,2, Tingbao Zhao³, Wenhui Xiong¹, Shaonan Wen⁴, Xiaoming Jin¹, Xiao-Ming Xu¹

¹Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Department of Anatomy and Cell Biology,Indiana University School of Medicine ²Department of Spinal Cord Injury and Repair, Trauma and Orthopedics Institute of Chinese PLA,General Hospital of Jinan Military Region ³Department of Orthopedics, Shandong Cancer Hospital,Shandong University ⁴Department of Neurobiology, Institute of Basic Medical Sciences,Academy Military Medical Sciences

Summary

Nous décrivons une procédure expérimentale pour mesurer l’activité neuronale à travers les fenêtres optiques doubles au-dessus corticies somatosensoriel primaire bilatérale (S1) en Thy1-GCaMP6s des souris transgéniques à l’aide de 2 photons (2p) microscopie in vivo.