In Vitro et In Vivo la détection des Mitophagy dans les cellules humaines, C. Eleganset la souris
17.4K vues
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Cité 32 fois
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08:40 min
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22 novembre 2017
DOI :10.3791/56301-v
22 novembre 2017
•17.4K vues
Evandro F. Fang1,6 , Konstantinos Palikaras2 , Nuo Sun3 , Elayne M. Fivenson1 , Ryan D. Spangler4 , Jesse S. Kerr1 , Stephanie A. Cordonnier1 , Yujun Hou1 , Eszter Dombi5 , Henok Kassahun6 , Nektarios Tavernarakis2,7 , Joanna Poulton5 , Hilde Nilsen6 , Vilhelm A. Bohr1,8
1Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 2Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology - Hellas, 3Center for Molecular Medicine, National Heart Lung and Blood Institute, National Institutes of Health, 4Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, 5Nuffield Department of Obstetrics and Gynaecology, University of Oxford, 6Department of Clinical Molecular Biology, University of Oslo and Akershus University Hospital, 7Department of Basic Sciences, Faculty of Medicine, University of Crete, 8Danish Center for Healthy Aging, University of Copenhagen
Mitophagy, le processus de compensation endommagée mitochondries, est nécessaire au maintien de l’homéostasie et de la santé mitochondriale. Cet article présente certaines de la mitophagy dernière méthodes de détection dans les cellules humaines, Caenorhabditis eleganset la souris.
Chapters in this video
0:05
Title
1:30
Detection of Mitophagy in Human Cells using mt-Keima Plasmid
3:12
Assessing Mitophagy using Co-localization Between DCT-1 and LGG-1 in C. elegans
5:36
Detection of Mitophagy in Mice
6:19
Results: Evaluation of Mitophagy in Human Cells, C. elegans, and Mice
7:57
Conclusion
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