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Bioengineering

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Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
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Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

Article DOI: 10.3791/56364-v • 10:22 min • November 29th, 2017
November 29th, 2017 •
*1,2, *1, 1, 1, 3, 1,2, 3, 1,2,4
1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
* These authors contributed equally

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résumé
Automatic Translation

This protocol efficiently studies mammalian cell division in 3D collagen matrices by integrating synchronization of cell division, monitoring of division events in 3D matrices using live-cell imaging technique, time-resolved confocal reflection microscopy and quantitative imaging analysis.

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Mammalian Cell Division 3D Matrices Quantitative Confocal Reflection Microscopy Cell Matrix Interactions Physiologically Relevant Efficient Approach Cell Biology Molecular Basis Normal Tissue Development Disease Tissue Development Transfection HEK-293 T-cells Cell Culture Medium Transfection Reagent Lentiviral Vector Plasmids Reduced Serum Medium
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