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JoVE Journal Behavior
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An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a Drosophila Model of Neurodegeneration

Article DOI: 10.3791/56507-v • 06:52 min • September 12th, 2017
September 12th, 2017 •
*1,2,3, *1,2,3, 1,2,3, 1,2,3, 1,2,3, 4,5, 1,2,3
1Research Center for Translational Medicine, Tongji University School of Medicine, 2Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, 3Department of Anatomy and Neurobiology, Tongji University School of Medicine, 4Shanghai Advanced Research Institute, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 5Sino-Danish College, University of Chinese Academy of Sciences, Chinese Academy of Sciences
* These authors contributed equally

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résumé
Automatic Translation

This step-by-step protocol analyzes Drosophila negative geotaxis behavior using an automated multi-cylinder system that hosts hundreds of flies and synchronizes their action by an electric motor. Upon synchronization, fly negative geotaxis behavior is assayed, digitally recorded, and analyzed using the self-designed RflyDetection software.

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Automated Rapid Iterative Negative Geotaxis Assay Adult Climbing Behavior Drosophila Model Neurodegeneration Locomotive Deficits Neuroscience Fly Climbing Activity Automated Analysis High Throughput Therapy Diagnosis Neurodegenerative Diseases Movement Ability Lifespan Age-dependent Neurodegeneration Fly Collection UAS-Dicer2 DDC-GAL4 Fly Virgins Genotypes
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