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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Article DOI: 10.3791/56814-v • 09:33 min • March 21st, 2018
March 21st, 2018 •
*1, *1, *1, 1, 2, 1
1Department of Chemistry, Capital Normal University, 2College of Life Sciences, Capital Normal University
* These authors contributed equally

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résumé
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A protocol for the in vitro selection and characterization of group-specific phthalic acid ester- binding DNA aptamers is presented. The application of the selected aptamer in an electrochemical aptasensor is also included.

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Phthalic Acid Ester-Binding DNA Aptamer Selection Characterization Application Electrochemical Aptasensor Hydrophobic Molecules Biosensor Development Affinity Measurements RNase-Free Water PCR Product Lambda Exonuclease Reaction Salt Concentration Ethanol Precipitation Isopropanol Micro-centrifuge Tubes Reaction Buffer Gentle Pipetting
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