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Primary Cell Cultures from the Mouse Retinal Pigment Epithelium
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Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

Article DOI: 10.3791/56997-v • 09:35 min • March 16th, 2018
March 16th, 2018 •
1, 1, 1, 2, 1,2
1Department of Ophthalmology, University of Pittsburgh School of Medicine, 2Wilmer Eye Institute, The Johns Hopkins University School of Medicine

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résumé
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The retinal pigment epithelium (RPE) is a multi-functional epithelium of the eye. Here we present a protocol to establish primary cell cultures derived from the murine RPE.

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Primary Cell Cultures Mouse Retinal Pigment Epithelium In Vitro Culture RPE Researchers Dissection Skills Mouse RPE Cell Cultures Simple And Feasible Procedure Isolation Of Mouse Eyeballs Euthanize Mice Obtaining Skills Angled Scissors 70% Ethanol PBS Petri Dish Connective Tissue Removal Dissecting Microscope
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