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Genetics

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Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

Article DOI: 10.3791/57080-v • 11:07 min • December 29th, 2017
December 29th, 2017 •
1, 1, 1, 1
1Department of Biological Sciences, University of Maryland

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Here, we describe a protocol for chromatin immunoprecipitation of modified histones from the budding yeast Saccharomyces cerevisiae. Immunoprecipitated DNA is subsequently used for quantitative PCR to interrogate the abundance and localization of histone post-translational modifications throughout the genome.

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Chromatin Immunoprecipitation ChIP Histone Modifications Saccharomyces Cerevisiae Chromatin Regulation Gene Expression Control Environmental Conditions Epitope Tags Antibodies YPD Medium Optical Density OD600 Culture Growth Formaldehyde Concentration
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