Mesure de la consommation d’oxygène mitochondriale dans les fibres perméabilisées de la drosophile à l’aide de petites quantités de tissu
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April 07, 2018
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The overall goal of this procedure is to evaluate mitochondrial respiration in the common fruit fly, Drosophila Melanogaster, using high resolution respirometry.This is achieved by first dissecting the Drosophila, to collect the thorax.The thoraces containing the flight muscles are then carefully permeabilized mechanically, with sharp forceps, and incubated with saponin, for further permeabilization.The thoraces are then transferred into the chambers of a respirometer, and mitochondrial oxygen consumption is measured with a combination of different substrates, uncouplers and inhibitors.Hi, I am Chlo
Summary
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Dans cet article, on décrit une méthode pour mesurer la consommation d’oxygène à l’aide de respirométrie haute résolution en thoraxes perméabilisées de drosophile. Cette technique nécessite une quantité minimale de tissu par rapport à la technique d’isolement mitochondrial classique et les résultats obtenus sont plus physiologiquement pertinents.
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Cite this Article
Simard, C. J., Pelletier, G., Boudreau, L. H., Hebert-Chatelain, E., Pichaud, N. Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue. J. Vis. Exp. (134), e57376, doi:10.3791/57376 (2018).
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