Experimental Protocol for Using <em>Drosophila</em> As an Invertebrate Model System for Toxicity Testing in the Laboratory

Elizabeth K. Peterson; Hugh E. Long

doi:10.3791/57450

Protocole expérimental pour l’utilisation de drosophile comme un système modèle d’invert...

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JoVE Journal Biology

Experimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory

Protocole expérimental pour l’utilisation de drosophile comme un système modèle d’invertébrés pour les essais de toxicité en laboratoire

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06:00 min

July 10, 2018

DOI: 10.3791/57450-v

Elizabeth K. Peterson^1,2, Hugh E. Long^1,2

¹Communities for Building Active STEM Engagement,Colorado State University-Pueblo, ²Department of Biology,Colorado State University-Pueblo

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Overview

This paper presents a detailed protocol for exposing Drosophila species to pollutants to study the effects on phenotypic outputs across developmental stages and generations.

Key Study Components

Area of Science

Neuroscience
Environmental Toxicology
Developmental Biology

Background

Drosophila serves as a model organism for studying genetic and environmental interactions.
Understanding the impact of pollutants is crucial for assessing ecological and health risks.
Research on multi-generational effects can provide insights into evolutionary adaptations.
Protocols for toxicant exposure are essential for reproducibility in research.

Purpose of Study

To investigate the impact of toxicants on fitness at various developmental stages.
To examine the effects of pollutant exposure across generations.
To provide a standardized protocol for researchers in the field.

Methods Used

Determine the 50% lethal dose of the compound for Drosophila.
Create a dose-response curve using established methods.
Prepare growth medium with the pollutant at the desired concentration.
Conduct experiments with undergraduate students to enhance engagement in research.

Main Results

Demonstrated the procedure for exposing Drosophila to environmental contaminants.
Provided insights into the phenotypic changes resulting from pollutant exposure.
Highlighted the importance of multi-generational studies in understanding toxic effects.
Engaged undergraduate students in hands-on research experiences.

Conclusions

The protocol offers a valuable tool for researchers studying environmental impacts on Drosophila.
Findings can inform broader ecological and health-related research.
Encouraging student involvement fosters future interest in scientific research.

Frequently Asked Questions

What is the significance of using Drosophila in this research?

Drosophila is a widely used model organism that allows for the study of genetic and environmental interactions.

How does pollutant exposure affect Drosophila?

Pollutant exposure can lead to various phenotypic changes and impact fitness across generations.

What are the steps involved in preparing the growth medium?

The growth medium is prepared by adding the pollutant to the water aliquot when making instant medium.

Why is it important to determine the 50% lethal dose?

Determining the 50% lethal dose helps establish safe exposure levels for experimental studies.

How can this research contribute to environmental science?

This research can provide insights into the ecological impacts of pollutants and inform risk assessments.

Dans cet article, nous fournissons un protocole détaillé d’exposer des espèces du genre Drosophila aux polluants dans le but d’étudier l’impact de l’exposition sur un éventail de sorties phénotypiques à différents stades de développement et de plusieurs générations.

Ce protocole peut être utilisé pour comprendre l’impact des substances toxiques sur la valeur adaptative à différents stades de développement et pour examiner l’effet de l’exposition aux polluants sur plusieurs générations. Deux étudiants de premier cycle de mon laboratoire de recherche qui font partie des communautés pour renforcer l’engagement actif des STIM à l’Université d’État du Colorado à Pueblo, Hugh Long et Tyler Harvey, feront la démonstration de la procédure. Pour commencer à travailler avec un contaminant environnemental d’intérêt, déterminez d’abord la dose létale de 50 % du composé drosophilie.

Utiliser des méthodes établies pour créer une courbe dose-réponse. Ensuite, préparez le milieu de culture avec le composé à la concentration souhaitée. Par exemple, ajoutez le composé dans l’aliquote d’eau lors de la fabrication d’un milieu instantané.

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