Un modèle de souris chimériques du foie humain de l’hypercholestérolémie utilisant des hépatocytes de dérivés de cellules souches pluripotentes induites

7.6K vues

Cité par 5

10:56 min

15 septembre 2018

10.3791/57556-v

15 septembre 2018

7.6K vues

Jiayin Yang^*¹^,²^,⁶ , Lai-Yung Wong^*² , Xiao-Yu Tian^*³ , Rui Wei¹^,² , Wing-Hon Lai² , Ka-Wing Au² , Zhiwei Luo⁴^,⁵ , Carl Ward⁴^,⁵ , Wai-In Ho² , David P. Ibañez⁴^,⁵ , Hao Liu⁴^,⁵ , Xichen Bao⁴^,⁵ , Baoming Qin⁴^,⁵ , Yu Huang³ , Miguel A. Esteban⁴^,⁵^,⁷ , Hung-Fat Tse¹^,²^,⁶^,⁷

¹Department of Medicine, University of Hong Kong-Shenzhen Hospital, ²The Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, ³School of Biomedical Sciences, Institute of Vascular Medicine, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, ⁴Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical University, ⁵Laboratory of RNA, Chromatin, and Human Disease, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, ⁶Research Centre of Heart, Brain, Hormone, and Healthy Ageing, Li Ka Shing Faculty of Medicine, University of Hong Kong, ⁷Hong Kong-Guangdong Stem Cell and Regenerative Medicine Research Centre, University of Hong Kong and Guangzhou Institutes of Biomedicine and Health

^* These authors contributed equally

Nous présentons ici un protocole visant à créer un modèle de souris chimériques du foie humain de l’hypercholestérolémie familiale à l’aide des hépatocytes de dérivés de cellules souches humaines pluripotentes induites. Il s’agit d’un modèle précieux pour tester de nouvelles thérapies pour l’hypercholestérolémie.