Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

Daniel D. T. Andrews; Tamara A. Franz-Odendaal

doi:10.3791/57619

Méthode de Culture organotypique pour étudier le développement des tissus embryonnaires de poulet

JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

Méthode de Culture organotypique pour étudier le développement des tissus embryonnaires de poulet

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05:12 min

August 25, 2018

DOI: 10.3791/57619-v

Daniel D. T. Andrews¹, Tamara A. Franz-Odendaal¹

¹Department of Biology,Mount Saint Vincent University

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Overview

This article presents a protocol for organotypic culturing of embryonic chicken organs, specifically focusing on the eye. This method allows researchers to study the development of embryonic tissues in a controlled in vitro environment.

Key Study Components

Area of Science

Neuroscience
Developmental Biology
In Vitro Culturing Techniques

Background

Studying embryonic chicken eyes is challenging due to eyelid development and embryo movement.
Extra embryonic tissues complicate the analysis of eye development.
Using drugs and inhibitors can be lethal to the embryo, hindering research.
In vitro culturing techniques help isolate tissues for study.

Purpose of Study

To provide access to embryonic chicken eyes for developmental studies.
To enable the examination of complex developmental pathways.
To study interactions between various eye tissues during development.

Methods Used

Autoclaving materials for sterility.
Organotypic culturing of embryonic chicken tissues.
Isolation of the eye and its interacting tissues.
In-depth analysis of developmental pathways.

Main Results

The protocol allows for the study of the eye as a whole.
Interactions between the neural retina and retinal pigmented epithelium can be observed.
Research can be conducted without the lethal effects of compounds on the embryo.
Enhanced understanding of eye development processes.

Conclusions

The organotypic culturing protocol is effective for studying embryonic chicken eyes.
This method overcomes barriers present in traditional embryonic studies.
It provides a platform for future research on developmental biology.

Frequently Asked Questions

What is the main advantage of this culturing protocol?

It allows for the study of embryonic chicken eyes in a controlled environment, overcoming challenges faced in vivo.

How does this method improve the study of eye development?

By isolating the eye and its tissues, researchers can examine interactions and developmental pathways without lethal effects from compounds.

What tissues can be studied using this protocol?

The protocol allows for the study of the neural retina and retinal pigmented epithelium, among others.

Is sterility important in this protocol?

Yes, autoclaving materials ensures that all solutions and equipment are sterile, which is crucial for successful culturing.

What are the implications of this research?

This research can lead to a better understanding of eye development and potential applications in regenerative medicine.

Nous présentons ici un organotypique cultivant protocole pour cultiver des organes embryonnaires de poulet in vitro. En utilisant cette méthode, le développement des tissus embryonnaires de poulet peut être étudié, tout en conservant un haut degré de contrôle sur le milieu de culture.

L’objectif global de ce protocole est de fournir un accès aux yeux de poulet embryonnaires dans un cadre in vitro afin de permettre une étude approfondie des voies de développement complexes dans l’embryon. Chez le poulet embryonnaire, l’étude de l’œil est souvent difficile, en raison du développement des paupières, du mouvement de l’embryon et de la croissance de tissus embryonnaires supplémentaires. Des composés tels que les médicaments et les inhibiteurs peuvent également avoir des effets létaux sur l’embryon, ce qui rend difficile l’utilisation de ces composés pour moduler les voies génétiques.

Les progrès de la culture tissulaire in vitro ont permis aux scientifiques d’isoler ces tissus et d’éliminer bon nombre de ces barrières susmentionnées. Notre protocole est avantageux en ce sens qu’il nous permet d’étudier l’œil dans son ensemble, y compris tous les tissus qui ont le potentiel d’interagir au cours du développement, tels que la rétine neurale et l’épithélium pigmenté rétinien. Commencez par autoclaver les matériaux pour vous assurer que toutes les solutions et tous les équipements sont stériles avant la procédure.

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