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Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes
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Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes

Article DOI: 10.3791/57733-v • 10:46 min • October 7th, 2018
October 7th, 2018 •
1, 2, 1,2
1Department of Clinical Experimental Research, Glostrup Research Institute, 2Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University

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We demonstrate three different tissue preparation techniques for immunohistochemical visualization of rat retinal microvascular pericytes, i.e., cryo-sections, whole-mounts, and hypotonic isolation of the vascular network.

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Retinal Cryo-sections Whole-Mounts Hypotonic Isolated Vasculature Preparations Immunohistochemical Visualization Microvascular Pericytes Ophthalmology Field Pericyte Proliferation Pericyte Migration Visualization Options Karin Dreisig Frank W. Blixt Post-docs Laboratory Enucleate The Eye Scalpel Rat Eyelid Forceps Connective Tissue Muscular Tissue 4% Formaldehyde Phosphate-buffered Saline Corneal Limbus Sodium Buffer Dissection Scissors Cryo-protect The Tissue Sorenson's Phosphate Buffer Sucrose Concentration Yazulla Medium Gelatine From Porcine Skin Albumin From Chicken Egg White
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