Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / A Combinatorial Single-cell Approach to…
JoVE Journal
Genetics

Un abonnement à JoVE est nécessaire pour afficher ce contenu.

JoVE Journal Genetics
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
Journal (Genetics)
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
Journal (Genetics)
Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
 

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Article DOI: 10.3791/57831-v • 09:34 min • October 25th, 2018
October 25th, 2018 •
1, 1, 1, 1
1Division of Molecular Hematology, Lund Stem Cell Center, Lund University

Chapitres

résumé
Automatic Translation

Bulk gene expression measurements cloud individual cell differences in heterogeneous cell populations. Here, we describe a protocol for how single-cell gene expression analysis and index sorting by Florescence Activated Cell Sorting (FACS) can be combined to delineate heterogeneity and immunophenotypically characterize molecularly distinct cell populations.

Tags

Combinatorial Single-cell Approach Molecular Heterogeneity Immunophenotypic Heterogeneity Human Stem Cells Progenitor Populations Stem Cell Heterogeneity Cancer Cells Therapy Isolation Single-cell Gene Expression Analysis Vaccinosis Downstream Characterization Gene Priming Lineage Potential Lysis Buffer RNA/DNA-free Bench NP-40 DNTP DTT RNAse Inhibitor PCR Plate Adhesive Film FACS Machine Target Population Cell Sorting Stain Buffer
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter