Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / A Direct Force Probe for Measuring Mechanical…
JoVE Journal
Biochemistry

Un abonnement à JoVE est nécessaire pour afficher ce contenu.

JoVE Journal Biochemistry
A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
Journal (Biochemistry)
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
Constructing the Columns and the Mesh System for Leachate Collection to Study Mineral Weathering (Video) | JoVE
Journal (Biochemistry)
Constructing the Columns and the Mesh System for Leachate Collection to Study Mineral Weathering (Video) | JoVE
 

A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton

Article DOI: 10.3791/58038-v • 05:47 min • July 29th, 2018
July 29th, 2018 •
1, 1, 1
1Department of Chemical Engineering, University of Florida

Chapitres

résumé
Automatic Translation

In this protocol, we describe a micropipette method to directly apply a controlled force to the nucleus in a living cell. This assay allows interrogation of nuclear mechanical properties in the living, adherent cell.

Tags

Direct Force Probe Measuring Mechanical Integration Nucleus Cytoskeleton Nuclear Mechanics Force Deformation Cellular Components Nuclear Resistance Nuclear Coupling Cell Type Variation Technique Advantage Living Adherent Cell Nucleo-cytoskeleton NIH-3T3 Cells Hutchinson-Gilford Progerial Cells Glass Bottom Dish Fibronectin Coating Culture Cells DMEM Bovine Serum Penicillin-Streptomycin Confluency PBS Wash Growth Medium
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter