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Immunology and Infection

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Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Article DOI: 10.3791/58481-v • 08:33 min • November 17th, 2018
November 17th, 2018 •
1,2, 1,2, 1,2
1The Peter Gorer Department of Immunobiology, King's College London, 2The Francis Crick Institute

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Here we provide detailed protocols for the oral administration of antibiotics to mice, collection of fecal samples, DNA extraction and quantification of fecal bacteria by qPCR.

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Quantitative Polymerase Chain Reaction-based Analyses Murine Intestinal Microbiota Oral Antibiotic Treatment Research Cross Microbiota Interactions Microbial Dysbiosis Inflammatory Bowel Disease Antibiotic Treatment Regimes Weight Loss Oral Gavage Ampicillin Gentamicin Neomycin Metronidazole Vancomycin Water Syringe Gavage Needle Esophagus Pharynx Solution
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