Growing Magnetotactic Bacteria of the Genus <em>Magnetospirillum</em>: Strains MSR-1, AMB-1 and MS-1

Lucas Le Nagard; Viviana Morillo-L&#243;pez; Cecile Fradin; Dennis A. Bazylinski

doi:10.3791/58536

Croissance des bactéries magnétotactiques du genre Magnetospirillum: souches MSR-1, 1-AM...

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Growing Magnetotactic Bacteria of the Genus Magnetospirillum: Strains MSR-1, AMB-1 and MS-1

Croissance des bactéries magnétotactiques du genre Magnetospirillum: souches MSR-1, 1-AMB et MS-1

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10:07 min

October 17, 2018

DOI: 10.3791/58536-v

Lucas Le Nagard¹, Viviana Morillo-López², Cecile Fradin¹, Dennis A. Bazylinski²

¹Department of Physics & Astronomy,McMaster University, ²School of Life Sciences,University of Nevada Las Vegas

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Overview

This study presents a comprehensive method for cultivating several strains of magnetotactic bacteria, specifically Magnetospirillum gryphiswaldense MSR-1, M. magneticum AMB-1, and M. magnetotacticum MS-1, under optimized oxygen control in both liquid and semi-solid media. The method ensures reproducibility and defines crucial steps for maintaining the specific oxygen requirements essential for these organisms.

Key Study Components

Research Area

Microbiology
Magnetotactic bacteria
Environmental biology

Background

Magnetotactic bacteria navigate using magnetic fields and have strict oxygen requirements.
The need for controlled oxygen environments for successful cultivation.
Focus on freshwater species within the genus Magnetospirillum.

Methods Used

Protocol for growing three species of magnetotactic bacteria
Use of liquid and semi-solid media
Optimized gas flow techniques for oxygen control

Main Results

Successful growth of MSR-1, AMB-1, and MS-1 in optimized media.
Establishment of protocols ensuring sterile conditions to prevent contamination.
Demonstrated the efficiency of nitrogen bubbling techniques in media preparation.

Conclusions

The study establishes a reliable procedure for cultivating magnetospirilla.
Provides foundational techniques applicable to broader research involving magnetotactic bacteria.

Frequently Asked Questions

What are magnetotactic bacteria?

Magnetotactic bacteria are microorganisms that orient and navigate along magnetic fields, often utilizing magnetosomes, which are intracellular organelles containing magnetic minerals.

Why is oxygen control critical in culturing these bacteria?

These bacteria have specific oxygen requirements; therefore, controlling oxygen levels is essential for their growth and to study their biological properties.

What species of magnetotactic bacteria are studied?

The study focuses on Magnetospirillum gryphiswaldense strain MSR-1, M. magneticum strain AMB-1, and M. magnetotacticum strain MS-1.

What media types are used for cultivation?

Both liquid and oxygen concentration gradient semi-solid media are utilized for growing the strains.

How does this method contribute to future research?

This method sets a framework for reproducible studies and understanding the physiology of magnetotactic bacteria, paving the way for further research in environmental microbiology.

What is the significance of sterilization in the procedure?

Sterilization is crucial to avoid contamination from other bacterial species, which could compromise the integrity of the experimental results.

Can the protocol be adapted for other magnetotactic bacteria?

Yes, the protocol can be optimized to meet the specific oxygen and nutrient requirements of other magnetotactic bacteria.

Nous présentons une procédure pour la culture de plusieurs souches de Magnetospirillum dans deux différents types de milieux de culture. Magnetospirillum gryphiswaldense souche MSR-1 est cultivée en liquide et en gradient de concentration₂ O milieu semi solide alors que M. magneticum souche AMB-1 et M. magnetotacticum souche MS-1 sont cultivés en milieu liquide.

Cette méthode permet de cultiver trois espèces différentes de bactéries magnétoactiques appelées MSR-1, AMB-1 et MS-1. Toutes les espèces d’eau douce, et toutes du genre magnétospirillum. Il s’agit d’une première étape essentielle pour toute recherche reproductible impliquant ces organismes.

Les bactéries magnétoactiques ont des besoins en oxygène très spécifiques, c’est pourquoi elles ont développé un système de navigation magnétique. Il est donc très important de contrôler la quantité d’oxygène à leur disposition. Tout ce protocole a été optimisé pour quelques souches spécifiques de magnétospirille d’eau douce.

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