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En rensing og I Vitro aktivitet analysen for en (p) ppGpp Synthetase fra Clostridium difficile

Article DOI: 10.3791/58547-v • 09:53 min • November 3rd, 2018
November 3rd, 2018 •
1, 1, 1
1Department of Chemistry and Biochemistry, Old Dominion University

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Her beskriver vi en metode for rensing histidin-merket pyrophosphokinase enzymer og utnytte tynt lag kromatografi radiolabelled underlag og produkter til analysen for den enzymatiske aktiviteten i vitro. Enzym aktivitet analysen er bredt aktuelt noen kinase, nucleotide cyclase eller fosfor-transfer reaksjon som mekanisme inkluderer nukleotid trifosfat hydrolyse.

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Immunologi og infeksjon utstede 141: Protein rensing histidin merke (p) ppGpp tynt lag Ture radionuklidenes merking pyrophosphokinase phosphotransfer
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