Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Environment / Detectie van Tilapia Lake Virus met behulp van…
JoVE Journal
Environment
Author Produced

Ce contenu est en libre accès.

JoVE Journal Environment
Detectie van Tilapia Lake Virus met behulp van conventionele rechts-PCR en SYBR groene RT-qPCR
A CO2 Concentration Gradient Facility for Testing CO2 Enrichment and Soil Effects on Grassland Ecosystem Function
Journal (Environment)
A CO2 Concentration Gradient Facility for Testing CO2 Enrichment and Soil Effects on Grassland Ecosystem Function
Application of Two-spotted Spider Mite Tetranychus urticae for Plant-pest Interaction Studies
Journal (Environment)
Application of Two-spotted Spider Mite Tetranychus urticae for Plant-pest Interaction Studies
 
Click here for the English version

Detectie van Tilapia Lake Virus met behulp van conventionele rechts-PCR en SYBR groene RT-qPCR

Article DOI: 10.3791/58596-v • 10:55 min • November 10th, 2018
November 10th, 2018 •
1, 2, 2
1Institute of Veterinary Bacteriology Vetsuisse Faculty, University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University

Chapitres

résumé
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Dit protocol diagnose Tilapia Lake Virus (TiLV) in tilapia weefsels met behulp van de RT-PCR methodes. De hele methode is beschreven van weefsel dissectie totaal RNA extractie, gevolgd door de cDNA synthese en opsporing van TiLV door conventionele PCR of kwantitatieve PCR dsDNA bindend een bindende fluorescerende kleurstof gebruiken.

Tags

Milieuwetenschappen kwestie 141 Tilapia lake virus tilapia diagnose RT-PCR RT-qPCR orthomyxo-achtig virus
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter