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Immunology and Infection

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Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry

Article DOI: 10.3791/58848-v • 08:06 min • February 27th, 2019
February 27th, 2019 •
1, 1, 1, 1
1Department of Medical Cell Biology, Uppsala University

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Herein, we present a protocol to prepare single cells from murine thymus, pancreatic draining lymph node and spleen to further study these cells using flow cytometry. In addition, this protocol was used for determining the subsets of regulatory T cells using flow cytometry.

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Regulatory T Cell Subsets Murine Thymus Pancreatic Draining Lymph Node Spleen Flow Cytometry Immune Cells Cost-effective Technique Manual Technique Zhengkang Luo PhD Student Euthanized Mice Scintillation Phials Conical Tubes Hank's Balanced Salt Solution RPMI-1640 Red Blood Cells Ammonium Chloride HBSS
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