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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifikation af mediatorer af T-celle receptoren signalering via Screening af kemiske hæmmer biblioteker
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Identifikation af mediatorer af T-celle receptoren signalering via Screening af kemiske hæmmer biblioteker

Article DOI: 10.3791/58946-v • 08:49 min • January 22nd, 2019
January 22nd, 2019 •
*1, *2,3, *1, 1, 1,4
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Singapore Immunology Network, A*STAR, 3Curiox Biosystems, 4Department of Immunobiology, Rega Institute for Medical Research, Katholieke Universiteit (KU) Leuven
* These authors contributed equally

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Dette papir bruger en flow-flowcytometri-baserede assay til skærmen biblioteker af kemiske hæmmere for identifikation af hæmmere og deres mål, der har indflydelse på T-celle receptoren signalering. De metoder, der beskrives her kan også udvides til høj overførselshastighed screeninger.

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Immunologi og infektion spørgsmålet 143 Caspase-3 aktivering kinase hæmmere bibliotek screening TCR signalering T-celle aktivering thymocyte udvalg
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