-1::1
Simple Hit Counter
Skip to content

Products

Solutions

×
×
Sign In

FR

EN - EnglishCN - 简体中文DE - DeutschES - EspañolKR - 한국어IT - ItalianoFR - FrançaisPT - Português do BrasilPL - PolskiHE - עִבְרִיתRU - РусскийJA - 日本語TR - TürkçeAR - العربية
Sign In Start Free Trial

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
View All
JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

Biological Techniques
Biology
Cancer Research
Immunology
Neuroscience
Microbiology
JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduate courses

Analytical Chemistry
Anatomy and Physiology
Biology
Calculus
Cell Biology
Chemistry
Civil Engineering
Electrical Engineering
View All
JoVE Science Education

Visual demonstrations of key scientific experiments

Advanced Biology
Basic Biology
Chemistry
View All
JoVE Lab Manual

Videos of experiments for undergraduate lab courses

Biology
Chemistry

BUSINESS

JoVE Business

Video textbooks for business education

Accounting
Finance
Macroeconomics
Marketing
Microeconomics

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Authors

Teaching Faculty

Librarians

K12 Schools

Biopharma

Products

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduates

JoVE Science Education

Visual demonstrations of key scientific experiments

JoVE Lab Manual

Videos of experiments for undergraduate lab courses

BUSINESS

JoVE Business

Video textbooks for business education

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Solutions

Authors
Teaching Faculty
Librarians
K12 Schools
Biopharma

Language

French

EN

English

CN

简体中文

DE

Deutsch

ES

Español

KR

한국어

IT

Italiano

FR

Français

PT

Português do Brasil

PL

Polski

HE

עִבְרִית

RU

Русский

JA

日本語

TR

Türkçe

AR

العربية

    Menu

    JoVE Journal

    Behavior

    Biochemistry

    Bioengineering

    Biology

    Cancer Research

    Chemistry

    Developmental Biology

    Engineering

    Environment

    Genetics

    Immunology and Infection

    Medicine

    Neuroscience

    Menu

    JoVE Encyclopedia of Experiments

    Biological Techniques

    Biology

    Cancer Research

    Immunology

    Neuroscience

    Microbiology

    Menu

    JoVE Core

    Analytical Chemistry

    Anatomy and Physiology

    Biology

    Calculus

    Cell Biology

    Chemistry

    Civil Engineering

    Electrical Engineering

    Introduction to Psychology

    Mechanical Engineering

    Medical-Surgical Nursing

    View All

    Menu

    JoVE Science Education

    Advanced Biology

    Basic Biology

    Chemistry

    Clinical Skills

    Engineering

    Environmental Sciences

    Physics

    Psychology

    View All

    Menu

    JoVE Lab Manual

    Biology

    Chemistry

    Menu

    JoVE Business

    Accounting

    Finance

    Macroeconomics

    Marketing

    Microeconomics

Start Free Trial
Loading...
Home
JoVE Journal
Immunology and Infection
Photothérapie quotidienne avec lumière rouge pour réglementer Candida albicans biofilms ...
Photothérapie quotidienne avec lumière rouge pour réglementer Candida albicans biofilms ...
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Daily Phototherapy with Red Light to Regulate Candida albicans Biofilm Growth

Photothérapie quotidienne avec lumière rouge pour réglementer Candida albicans biofilms croissance

Full Text
8,307 Views
11:06 min
April 23, 2019

DOI: 10.3791/59326-v

Beatriz H D Panariello1, Bruna A Garcia2, Simone Duarte1

1Department of Cariology, Operative Dentistry and Dental Public Health,Indiana University School of Dentistry, 2Department of Restorative Dentistry,Ceará Federal University, School of Dentistry

AI Banner

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This study presents a protocol to evaluate the impact of red light therapy on the growth of Candida albicans biofilms. Utilizing a non-coherent red light device at a wavelength of 635 nm, the research aims to assess how phototherapy affects biofilm development over a 48-hour period.

Key Study Components

Area of Science

  • Microbiology
  • Fungal infections
  • Phototherapy

Background

  • Candida is a leading cause of fungal infections in immunocompromised individuals.
  • Biofilms are resistant to traditional therapies due to their protective matrix.
  • Phototherapy may offer a novel approach to disrupt biofilm growth.
  • The strain used has a well-characterized extracellular matrix.

Purpose of Study

  • To evaluate the effect of red light on Candida albicans biofilm growth.
  • To explore the potential of phototherapy as a treatment option.
  • To assess the clinical applicability of the red light device.

Methods Used

  • Application of a non-coherent red light device at 635 nm.
  • Assessment of biofilm growth over a 48-hour period.
  • Characterization of the extracellular matrix of the Candida strain.
  • Comparison with traditional therapeutic approaches.

Main Results

  • Red light application significantly interferes with biofilm growth.
  • Phototherapy shows promise for reducing treatment time.
  • Results indicate a potential new avenue for clinical applications.
  • Further studies are needed to confirm findings.

Conclusions

  • Red light therapy may enhance treatment efficacy against Candida biofilms.
  • Phototherapy could be a viable alternative to traditional therapies.
  • Future research should focus on optimizing treatment protocols.

Frequently Asked Questions

What is the wavelength of the red light used?
The red light device operates at a wavelength of 635 nm.
How long was the red light applied to the biofilms?
The red light was applied throughout the 48-hour growth period of the biofilms.
What is Candida albicans?
Candida albicans is a common fungal pathogen, particularly in immunocompromised individuals.
Why are biofilms resistant to traditional therapies?
Biofilms are resistant due to their protective extracellular matrix, which limits the penetration of therapeutic agents.
What are the advantages of using phototherapy?
Phototherapy may reduce treatment time and enhance the effectiveness of antifungal treatments.
Is the red light device applicable to other microorganisms?
Yes, the device has been successfully applied to other planktonic microorganisms and biofilms.

Nous présentons ici un protocole afin de mesurer le résultat de l’application de la lumière rouge sur la croissance de Candida albicans biofilms. Un dispositif rouge non cohérentes avec la longueur d’onde de 635 nm et densité d’énergie de 87,6 J·cm-2 a été appliqué tout au long de la croissance de Candida albicans biofilms pendant 48 h.

Candida est la cause la plus fréquente d’infection fongique disséminée chez les hôtes immunocompromisés. L’incapacité des agents thérapeutiques à infiltrer complètement le biofilm en raison de la matrice est l’une des raisons pour lesquelles les biofilms résistent aux thérapies traditionnelles. Notre protocole évalue comment la photothérapie, à l’aide de la lumière rouge, interfère avec la croissance et l’arrangement des biofilms Candida albicans.

Les méthodes appliquées sont bien établies, puisque la souche utilisée dans cette étude a eu sa matrice extracellulaire caractérisée et le dispositif léger a été appliqué avec succès à d’autres suspensions planctoniques de micro-organisme et biofilms. Le plus grand avantage de cet appareil est la réduction du temps de traitement, ce qui le rend plus visible pour les applications cliniques. Lors de la culture Candida albicans être conscient que les anticorps peuvent être acquis.

View the full transcript and gain access to thousands of scientific videos

View the full transcript and gain access to thousands of scientific videos

Sign In Start Free Trial

Explore More Videos

Immunologie et Infection numéro 146 biofilm Candida albicans photothérapie lumière rouge matrice extracellulaire polyssacharides

Related Videos

Candida albicans biofilm Chip (Ca BChip) pour criblage à haut débit des médicaments antifongiques

08:05

Candida albicans biofilm Chip (Ca BChip) pour criblage à haut débit des médicaments antifongiques

Related Videos

14.2K Views

Clarification et imagerie Candida albicans Biofilms

11:09

Clarification et imagerie Candida albicans Biofilms

Related Videos

13.7K Views

Thérapie photodynamique médiée par rose Bengal pour inhiber Candida albicans

04:48

Thérapie photodynamique médiée par rose Bengal pour inhiber Candida albicans

Related Videos

3.8K Views

Essai de réduction soluble à base de tétrazolium pour évaluer l’effet des anticorps sur les biofilms de Candida tropicalis

06:50

Essai de réduction soluble à base de tétrazolium pour évaluer l’effet des anticorps sur les biofilms de Candida tropicalis

Related Videos

3.7K Views

THÉRAPIE PHOTODYNAMIQUE ANTIMICROBIENNE MÉDIÉE PAR LES CURCUMINOÏDES SUR UN MODÈLE MURIN DE CANDIDOSE BUCCALE

06:39

THÉRAPIE PHOTODYNAMIQUE ANTIMICROBIENNE MÉDIÉE PAR LES CURCUMINOÏDES SUR UN MODÈLE MURIN DE CANDIDOSE BUCCALE

Related Videos

1.5K Views

Induction de petites colonies chez Candida glabrate via la thérapie photodynamique médiée par Rose Bengal

09:06

Induction de petites colonies chez Candida glabrate via la thérapie photodynamique médiée par Rose Bengal

Related Videos

1.2K Views

Une plaque de microtitrage à 96 puits à base Méthode de formation suivi et les tests de sensibilité antifongique des Candida albicans Les biofilms

07:19

Une plaque de microtitrage à 96 puits à base Méthode de formation suivi et les tests de sensibilité antifongique des Candida albicans Les biofilms

Related Videos

46.5K Views

Plaque de microtitrage biofilm essai de formation

03:57

Plaque de microtitrage biofilm essai de formation

Related Videos

115.7K Views

Etudier communautés microbiennes In Vivo: Un modèle d'interaction hôte intermédiaire Entre Candida albicans Et Pseudomonas aeruginosa Dans les Airways

06:43

Etudier communautés microbiennes In Vivo: Un modèle d'interaction hôte intermédiaire Entre Candida albicans Et Pseudomonas aeruginosa Dans les Airways

Related Videos

9.5K Views

Venez sur le côté de la lumière: In Vivo surveillance des Infections à Pseudomonas aeruginosa Biofilm dans les plaies chroniques dans un modèle murin glabres diabétique

09:15

Venez sur le côté de la lumière: In Vivo surveillance des Infections à Pseudomonas aeruginosa Biofilm dans les plaies chroniques dans un modèle murin glabres diabétique

Related Videos

14.2K Views

JoVE logo
Contact Us Recommend to Library
Research
  • JoVE Journal
  • JoVE Encyclopedia of Experiments
  • JoVE Visualize
Business
  • JoVE Business
Education
  • JoVE Core
  • JoVE Science Education
  • JoVE Lab Manual
  • JoVE Quizzes
Solutions
  • Authors
  • Teaching Faculty
  • Librarians
  • K12 Schools
  • Biopharma
About JoVE
  • Overview
  • Leadership
Others
  • JoVE Newsletters
  • JoVE Help Center
  • Blogs
  • JoVE Newsroom
  • Site Maps
Contact Us Recommend to Library
JoVE logo

Copyright © 2026 MyJoVE Corporation. All rights reserved

Privacy Terms of Use Policies
WeChat QR code