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Renal Subcapsular Transplantation of 2'-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

Article DOI: 10.3791/59657-v • 07:39 min • July 19th, 2019
July 19th, 2019 •
1,3,4, 1,3,4, 2,3,4, 1,3,4, 1, 2, 1,3,4, 1,3,4
1The First Affiliated Hospital, Jinan University, 2Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Jinan University, 3The Institute of Clinical Medicine, Jinan University, 4Joint institute of Metabolic Medicine Between Jinan University and University of Hong Kong

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résumé
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We provide a simple and efficient method to transplant 2’-deoxyguanosine treated E18.5 thymus into the renal capsule of a nude mouse. This method should aide in the study of both thymic epithelial cells function and T cells maturation.

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Renal Subcapsular Transplantation 2'-deoxyguanosine Treatment Murine Embryonic Thymus Nude Mice Thymus Epithelial Cell Function T-cell Maturation Isolation And Culture Mouse 18.5 Thymus Renal Capsule Transplantation Developmental Stages Tissue Transplantation Case Stamps Visualization Sacrifice Embryonic Day 18.5 Pregnant Mouse V-shaped Cut Abdomen Bladder Uterus Mesometrium Cervical And Vaginal Areas Petri Dish Cold PBS Decidual Tissues Umbilical Cord Embryo Transfer
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