Isolement et culture des cellules endothéliales aortiques primaires des porcs miniatures

8.4K vues

Cité par 6

06:38 min

14 août 2019

10.3791/59673-v

14 août 2019

8.4K vues

Yanli Zhao¹^,²^,³ , Chengjiang Zhao⁴ , David K.C. Cooper⁵ , Ying Lu² , Kewang Luo⁶ , Huiyun Wang³ , Pengfei Chen³ , Changchun Zeng³ , Shaodong Luan¹ , Lisha Mou² , Hanchao Gao¹^,²^,³

¹Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ²Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, ³Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ⁴Department of Endocrinology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ⁵Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, ⁶People's Hospital of Longhua

Une méthode enzymatique efficace pour l'isolement des cellules endothéliales aortiques primaires porcines (pAECs) des porcs miniatures est décrite. Les pAECs primaires isolés peuvent être employés pour étudier la réponse immunisée et de coagulation dans la xénotransplantation.