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Immunology and Infection

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JoVE Journal Immunology and Infection
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
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Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Article DOI: 10.3791/59809-v • 09:07 min • August 22nd, 2019
August 22nd, 2019 •
1, 1, 1, 2, 1
1World Without Disease Accelerator, Janssen Pharmaceutical Companies of Johnson and Johnson, 2Janssen Prevention Center, Janssen Pharmaceutical Companies of Johnson and Johnson

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The BSelex method to identify and recover individual antigen-specific antibodies from human peripheral blood mononuclear cells combines flow cytometry with single cell PCR and cloning.

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Single-cell Screening Method Selection And Recovery Antibodies Desired Specificities Enriched Human Memory B Cell Populations Immune Repertoires Antigen-specific B Cells Natively-paired Heavy And Light Chains Cloning Antigen Testing Mike Morton Technician Thawing And Recovery PBMC Collection Centrifugation CD22-positive B Cells Positive Selection CD22 Microbeads Vax Buffer Concentration IgG CD19 CD27 Antibody Cocktail Negative Control Dual-labeled Biotin Streptavidin Tetramers Sort Tube Volume
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