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A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells
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A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells

Article DOI: 10.3791/59881-v • 05:58 min • September 20th, 2019
September 20th, 2019 •
1, 1, 1, 1,2,3
1Department of Physiology, University of Ulsan College of Medicine/Asan Medical Center, 2Asan Medical Center, 3Asan Medical Institute of Convergence Science and Technology

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résumé
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Due to the spectral overlapping of the excitation and emission wavelengths of NADH and fura-2 analogs, the signal interference from both chemicals in live cells is unavoidable during quantitative measurement of [Ca2+]. Thus, a novel online correction method of NADH signal interference to measure [Ca2+] was developed.

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Nicotinamide Adenine Dinucleotide NADH Ca2+ Measurement Fura-2-Analog UV Excitable Plural Probes Signal Contamination Correction Method True Signal Extraction Research Assistant Professor Myocytes Fura-2-FFAM Diluting Solution Incubation Culture Medium Isobestic Fura-2-ffa Point Identification Microscope Stage Cell Culture Target Cell Saponin Solution Excitation Scan
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