JoVE Journal
Genetics
Genetics
Un abonnement à JoVE est nécessaire pour afficher ce contenu.
Chapitres
résumé
Reciprocal hemizygosity via sequencing (RH-seq) is a powerful new method to map the genetic basis of a trait difference between species. Pools of hemizygotes are generated by transposon mutagenesis and their fitness is tracked through competitive growth using high-throughout sequencing. Analysis of the resulting data pinpoints genes underlying the trait.