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Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
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Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Article DOI: 10.3791/60019-v • 07:26 min • July 29th, 2019
July 29th, 2019 •
1,2, 2, 2, 2, 2, 1,2, 1,2, 1,2
1State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, 2Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University

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We present a circular RT-PCR-based strategy by combining circular RT-PCR, quantitative RT-PCR, RNA 5' polyphosphatase-treatment, and Northern blot. This protocol includes a normalization step to minimize the influence of unstable 5' triphosphate, and it is suitable for discriminating and mapping the primary and processed transcripts stably accumulated in maize mitochondrion.

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Discrimination Mapping Primary Transcripts Processed Transcripts Maize Mitochondria Circular RT-PCR-based Strategy RNA Normalization Five Prime Triphosphate Plastids Plant Mitochondria Developing Kernels Extraction Buffer Filter Cloth Centrifuge Supernatant Wash Buffer Mitochondrial Pellet RNA Extraction Polyphosphatase Treatment
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