Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / A Cryo-pulverization Protocol for Processing…
JoVE Journal
Immunology and Infection

Un abonnement à JoVE est nécessaire pour afficher ce contenu.

JoVE Journal Immunology and Infection
A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model
Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
Journal (Immunology and Infection)
Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
Pseudomonas aeruginosa Induced Lung Injury Model
Journal (Immunology and Infection)
Pseudomonas aeruginosa Induced Lung Injury Model
 

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Article DOI: 10.3791/60229-v • 11:03 min • October 30th, 2019
October 30th, 2019 •
1, 1, 1, 1, 1, 1
1Immunology, Janssen Research & Development, Inc.

Chapitres

résumé
Automatic Translation

A cryogenic pulverization method to process murine paws using a liquid nitrogen freezer mill was developed to improve the yield and quality of RNA or protein extracted from the tissues and enable the analysis of molecular profiles associated with inflammatory responses.

Tags

Cryo-pulverization Protocol Mouse Paws Tissue Inflammation Collagen Induced Arthritis Model Molecular Pathways Evaluation Active Pharmaceuticals Local Tissues Arthritis Research Weight-bearing Joints Mechanical Disruption Cryogenically Controlled Environment Proteomic Profiling Transcriptional Profiling Molecular Signatures Homogenous Powder Temperature Isolation Procedure High-quality RNA Molecular Characterization Research Field
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter