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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
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Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria

Article DOI: 10.3791/60359-v • 08:27 min • October 31st, 2019
October 31st, 2019 •
1, 1
1Public Health Research Institute, New Jersey Medical School, Rutgers, The State University of New Jersey

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Automatic Translation

Mycobacterium tuberculosis shows increased production and release of extracellular vesicles in response to low iron conditions. This work details a protocol for generating low iron conditions and methods for the purification and characterization of mycobacterial extracellular vesicles released in response to iron deficiency.

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Isolation Characterization Extracellular Vesicles Iron-limited Mycobacteria Low Iron Conditions Membrane Vesicles Culture Filtrate Biochemical Assays Functional Assays Mycobacterium Tuberculosis Mycobacterium Smegmatis BSL-2 Facilities Minimal Medium Monopotassium Phosphate L-asparagine Glycerol Dextrose PH Adjustment Metal Chelating Resin Magnetic Stir Bar Filtration Zinc Chloride Magnesium Sulfate
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