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Immunology and Infection

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JoVE Journal Immunology and Infection
Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis
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Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis

Article DOI: 10.3791/61133-v • 09:02 min • February 20th, 2021
February 20th, 2021 •
1, 2, 2, 2, 2,3
1Department of Infection Biology, London School of Hygiene and Tropical Medicine, 2Institut Pasteur, Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence (CIBU), 3Cellule Pasteur, Université de Paris

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Reported here is a protocol for the quantification of infectious viral particles using real-time monitoring of electrical impedance of infected cells. A practical application of this method is presented by quantifying influenza A virus decay under different physicochemical parameters mimicking environmental conditions.

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Monitoring Influenza Virus Survival Host Real-time Cell Analysis Particle Quantification Biology Studies Viral Data Technique Traditional Measurement Real-time Data Confidence Interval Labor-intensive Endpoints Viruses Cytopathic Effect Quentin Grassin Laboratory Technician H1N1 Viruses MDCK Cells Tissue Culture Flasks Standard Protocols Incubate Confluence Wash Cells PBS Control Flask Influenza A Virus Stock Experimental Concentration Virus Propagation Medium Multiplicity Of Infection
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