Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Click-Chemistry Based Fluorometric Assay for…
JoVE Journal
Biochemistry

Un abonnement à JoVE est nécessaire pour afficher ce contenu.

JoVE Journal Biochemistry
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
A Rapidly Incremented Tethered-Swimming Maximal Protocol for Cardiorespiratory Assessment of Swimmers
Journal (Biochemistry)
A Rapidly Incremented Tethered-Swimming Maximal Protocol for Cardiorespiratory Assessment of Swimmers
Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
Journal (Biochemistry)
Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
 

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Article DOI: 10.3791/61146-v • 07:37 min • May 13th, 2020
May 13th, 2020 •
1, 1, 1
1Institut Pasteur, Unité Biologie et génétique de la paroi bactérienne, Paris, France ; CNRS, UMR 2001 « Microbiologie intégrative et Moléculaire », Paris, France ; INSERM, Équipe Avenir, Paris, France

Chapitres

résumé
Automatic Translation

Presented here is a sensitive fluorescence assay to monitor apolipoprotein N-acyltransferase activity using diacylglyceryl peptide and alkyne-phospholipids as substrates with click-chemistry.

Tags

Click-Chemistry Fluorometric Assay Apolipoprotein N-acyltransferase Enzyme Characterization High-Throughput Screening HDS Format Inhibitors Protocol Sensitive Assay Screening Purposes Potential Novel Antibiotics Mixing Vortexing Sonication Nonhomogeneous Lipid Detergent Containing Suspensions Reagent Mixture Alkyne POPE FSL-1-biotin LNT Reaction Buffer Triplicate Reagent Mixtures Sonication Ultrasonic Water Bath Incubation Active LNT Inactive LNT Concentration Thermal Mixer Heated Lid Stock Solution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter