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Developmental Biology

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Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses

Article DOI: 10.3791/61800-v • 09:59 min • October 7th, 2020
October 7th, 2020 •
1, 1, 1, 1, 3,4, 1,2, 3,4, 1, 1
1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit, University of Turku, Turku, Finland, 2Department of Pediatrics, Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute, Monash University, Melbourne, VIC 3800, Australia

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This protocol describes transillumination-assisted microdissection of segments of adult mouse seminiferous tubules representing specific stages of seminiferous epithelial cycle, and cell types therein, and subsequent immunostaining of squash preparations and intact tubule segments.

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Transillumination-assisted Dissection Mouse Seminiferous Epithelial Cycle Stage-specific Dissection Immunostaining Analyses Spermatogenesis Transillumination Patterns Opeyemi Olotu Ph.D Candidate Adult Male Mouse V-shaped Incision Epididymal Fat Pad Testes Dissection Tunica Albuginea Decapsulated Seminiferous Tubules Sterile PBS
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