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An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Article DOI: 10.3791/61925-v • 09:20 min • May 4th, 2021
May 4th, 2021 •
1, 1, 1, 1, 1
1Université Côte d’Azur, Inserm, C3M, Team Cellular and Molecular Pathophysiology of Obesity and Diabetes, Nice, France

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Presented here is a protocol to deliver oligonucleotides such as small-interfering RNA (siRNA), micro-RNA mimics (miRs), or anti-micro-RNA (anti-miR) into mature adipocytes to modulate protein and micro-RNA expression.

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Adipocyte Cell Culture Model Protein Modulation Micro-RNA Modulation Adipocyte Function Reverse-transfection Method Oligonucleotide Transfection 3T3-L1 Adipocytes Human Preadipocytes Cell Culture Incubator DMEM Glucose Newborn Calf Serum Penicillin And Streptomycin IBMX Dexamethasone Insulin Rosiglitazone
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