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En adipocytcellekulturmodel til undersøgelse af protein- og mikro-RNA-gradueringens indvirkning på adipocytfunktionen

Article DOI: 10.3791/61925-v • 09:20 min • May 4th, 2021
May 4th, 2021 •
1, 1, 1, 1, 1
1Université Côte d’Azur, Inserm, C3M, Team Cellular and Molecular Pathophysiology of Obesity and Diabetes, Nice, France

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Præsenteret her er en protokol til at levere oligonukleotider såsom små-interfererende RNA (siRNA), mikro-RNA efterligner (miRs), eller anti-mikro-RNA (anti-miR) i modne adipocytter til at modulere protein og mikro-RNA udtryk.

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Biologi Udgave 171 adipocytter cellekultur transfection mikro-RNA (miR) lille forstyrrende RNA (siRNA)
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