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A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish

Article DOI: 10.3791/61981-v • 11:24 min • June 30th, 2021
June 30th, 2021 •
1,3, 1,2,3
1Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine, 3Department of Genetics, Albert Einstein College of Medicine

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Here, we present the protocol for 3-D tissue culture of the zebrafish posterior body axis, enabling live study of vertebrate segmentation. This explant model provides control over axis elongation, alteration of morphogen sources, and subcellular resolution tissue-level live imaging.

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3-D Tail Explant Culture Vertebrate Segmentation Zebrafish Somite Development Transparency Fish System Water Mating Tank Strainer Embryos Petri Dish Dissection Tools Chamber Cuts Cell Culture Media Calcium Chloride Antibiotic Solution Fetal Bovine Serum Tissue Culture Medium De-yoking Coverslip Growth Medium Slide Chamber Microscope Laser Channels
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