Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (<em>Oryzias latipes</em>)

Muhammad Rahmad Royan; Shinji Kanda; Daichi Kayo; Weiyi Song; Wei Ge; Finn-Arne Weltzien; Romain Fontaine

doi:10.3791/62006

Gonadectomie et procédures de prélèvement sanguin dans le modèle de téléostéen de petite taille M...

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Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)

Gonadectomie et procédures de prélèvement sanguin dans le modèle de téléostéen de petite taille Medaka japonais (Oryzias latipes)

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05:31 min

December 11, 2020

DOI: 10.3791/62006-v

Muhammad Rahmad Royan¹, Shinji Kanda², Daichi Kayo³, Weiyi Song⁴, Wei Ge⁴, Finn-Arne Weltzien¹, Romain Fontaine¹

¹Physiology Unit, Faculty of Veterinary Medicine,Norwegian University of Life Sciences, ²Laboratory of Physiology, Atmosphere and Ocean Research Institute,The University of Tokyo, ³Department of Biological Sciences, Graduate School of Science,The University of Tokyo, ⁴Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences,University of Macau

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Overview

The article presents a quick protocol for gonadectomizing Japanese medaka (Oryzias latipes) and sampling blood to explore the role of sex steroids in animal physiology. This method allows researchers to effectively investigate the physiological functions of gonads and how these relate to neuroendocrine plasticity in teleosts and vertebrates.

Key Study Components

Research Area

Gonadectomy techniques in teleost fish
Investigation of sex steroids' physiological roles
Neuroendocrine system plasticity

Background

Japanese medaka is a widely used model in research
Understanding gonads' functions is critical for studying vertebrate physiology
Previous techniques lacked high survival rates

Methods Used

Protocols for ovariectomy and orchidectomy
Japanese medaka (Oryzias latipes) as the biological model
Blood sampling techniques and hormone analysis

Main Results

100% survival rate in females and 94% in males post-surgery
Significantly lower estrogen (E2) levels in ovariectomized fish compared to sham-operated fish
Reduced 11-ketotestosterone (11-KT) levels in orchidectomized males

Conclusions

This study demonstrates an effective method for gonadectomy with implications for hormone research
Findings highlight the role of sex steroids in the physiology of small teleost models

Frequently Asked Questions

What is the significance of gonadectomy in teleosts?

It allows researchers to study the physiological function of gonads and the impact of sex steroids on various biological processes.

Why is Japanese medaka commonly used in research?

Its small size, ease of handling, and well-characterized genetics make it ideal for laboratory studies.

How does this protocol ensure high survival rates?

Proper training in surgical techniques and careful execution of the procedure contribute to the high survival rate of the fish.

What are the consequences of gonad removal in fish?

Gonad removal alters hormone levels, affecting various physiological functions, including reproductive performance.

Can this method be adapted for other fish species?

While optimized for medaka, aspects of the protocol could be adapted for other small teleost species with proper adjustments.

What are the potential applications of this research?

It may provide insights into fish physiology, evolutionary biology, and signaling pathways related to sex steroids.

Are there any ethical considerations for this procedure?

As with all animal research, proper ethical guidelines must be followed to ensure the welfare of the fish used in experiments.

L’article décrit un protocole rapide pour gondécatomiser et prélassez le sang du petit poisson téléostéen, en utilisant le medaka japonais(Oryzias latipes)comme modèle, pour étudier le rôle des stéroïdes sexuels dans la physiologie animale.

Cette méthode aidera à répondre à des questions clés concernant la fonction physiologique des gonades, y compris le rôle des stéroïdes sexuels dans la plasticité et la régulation du système neuroendocrinien chez les téléostéens et, dans une plus large mesure, chez les vertébrés. Ce protocole permet de réaliser une gonadectomie à un taux de réussite élevé chez les petits modèles de médeka téléostéen, qui est largement utilisé dans le milieu de la recherche. Afin de réussir et d’atteindre un taux de survie élevé, cette technique nécessite une formation appropriée, notamment pour évaluer les gonades et suturer les poissons.

Commencez par retirer le poisson de la solution anesthésique et placez-le horizontalement sur le côté sous un microscope de dissection. Pour effectuer l’ovariectomie chez les femelles, grattez les écailles dans la zone d’incision. Faites doucement une incision de deux à 2,5 millimètres entre les côtes et les nageoires pelvienne et anale à l’aide d’une lame de rasoir.

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