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Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Article DOI: 10.3791/62008-v • 09:08 min • December 22nd, 2020
December 22nd, 2020 •
*1,2, *1,2, *1, 1, 1, 1,2, 3, 1, 1, 4,5, 1,5
1The Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York, 2The City College of New York, CUNY, 3The Bronx High School of Science, 4The Graduate Center - Advanced Science Research Center, Structural Biology Initiative, The City University of New York, 5The Graduate Center - Advanced Science Research Center, MALDI MS Imaging Joint Core Facility, The City University of New York
* These authors contributed equally

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résumé
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The goal of this protocol is to provide detailed guidance on the sample preparation when planning for experiments using MALDI MSI to maximize metabolic and molecular detection in biological samples.

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Sample Preparation Metabolic Profiling MALDI Mass Spectrometry Imaging Metabolomics Relative Metabolite Abundance Metabolite Distribution MALDI Imaging C2 Labeling Tissue Harvesting Liquid Nitrogen Aluminum Foil Boat Polystyrene Box Tissue Freezing Cryostat Sectioning Tissue Conductivity Testing Indium Tin Oxide Coated Glass Slides Labeling Cryostat Temperature
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