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Developmental Biology

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JoVE Journal Developmental Biology
Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts
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Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts

Article DOI: 10.3791/62021-v • 05:58 min • February 15th, 2021
February 15th, 2021 •
*1, *1, 1, 1, 1, 2, 1, 1
1School of Public Health, Soochow University, 2Toxicology, Risk Assessment and Research Division, Texas Commission on Environmental Quality
* These authors contributed equally

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résumé
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This protocol uses an immunofluorescence assay to detect PM2.5-induced DNA damage in the dissected hearts of zebrafish embryos.

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Immunofluorescence DNA Damage PM2.5 Zebrafish Embryo Hearts Target Proteins Expression Detection Stereo Microscope Heart Malformations Pericardial Edema Altered Looping Size Decrease Malformation Rates One Way ANOVA Turkey's Multiple Comparison Test MS222 Anesthesia Heartbeats Image J Software Heart Rates Quantification Dissected Hearts Fixative Solution Paraformaldehyde Hydrophobic Barrier Circle Incubation PBST Wash
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