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Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse

Article DOI: 10.3791/62058-v • 05:16 min • December 22nd, 2020
December 22nd, 2020 •
1,2,3, 1,2, 1,2,3, 1,3, 1,2, 1,2, 1,2,3, 1,2,3, 1,2,3
1University Hospital Munich, Department of Medicine I, Ludwig Maximilians University (LMU) Munich, 2Institute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig Maximilians University (LMU) Munich, 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance

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We provide a step-by-step protocol for whole-mount immunofluorescence staining of the sinoatrial node (SAN) and atrioventricular node (AVN) in murine hearts.

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Whole-mount Immunofluorescence Staining Confocal Imaging 3D Reconstruction Sinoatrial Node Atrioventricular Node Mouse Micro Dissection Microscopy Sinus Node AV Node 3D Localization Morphology Tissue Preservation Heart Puncture PBS Injection Dissection Dish Orientation Determination Heart Immobilization Non-cardiac Tissue Removal Intercaval Region Exposure
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