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Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Article DOI: 10.3791/62213-v • 10:08 min • February 24th, 2021
February 24th, 2021 •
1, 2,3, 4, 1, *2, *4
1University Bordeaux, INSERM, LAMC, U1029, 33600, Pessac, France, 2Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, Bordeaux, France, 3Alvéole, Bordeaux, France, 4University Bordeaux, CNRS, IBGC, UMR5095, 33000, Bordeaux, France
* These authors contributed equally

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Here, we present an easy-to-use co-culture assay to analyze glioblastoma (GBM) migration on patterned neurons. We developed a macro in FiJi software for easy quantification of GBM cell migration on neurons, and observed that neurons modify GBM cell invasive capacity.

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Co-culture Glioblastoma Stem-like Cells Patterned Neurons Migration Cellular Interactions Invasive Profile Reproducibility Clinical Diagnosis Clinical Outcome Migrating Cells Fibroblasts Immune Cells Micropatterning Substrate Triethoxysilane PEG-SVA Gel Deposition PLPP Ethanol Glass Slide Micropatterning
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