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Immunology and Infection

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JoVE Journal Immunology and Infection
Måling av pH, redokskjemier og nedbrytende kapasitet for makropinosomer ved hjelp av dual-fluorofor ratiometrisk mikroskopi
Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
Journal (Immunology and Infection)
Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness
Journal (Immunology and Infection)
A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness
 
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Måling av pH, redokskjemier og nedbrytende kapasitet for makropinosomer ved hjelp av dual-fluorofor ratiometrisk mikroskopi

Article DOI: 10.3791/62733-v • 07:31 min • August 19th, 2021
August 19th, 2021 •
1,2, 2,3
1Department of Biochemistry & Microbiology, Faculty of Science, University of Victoria, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Science, University of Calgary, 3Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases, University of Calgary

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Vi beskriver protokoller for måling av pH, oksidative hendelser og proteinfordøyelse i individuelle makropinosomer i levende celler. Det legges vekt på metrisk mikroskopi med dobbel fluorofor og fordelene ved populasjonsbaserte teknikker.

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Immunologi og infeksjon utgave 174 makropinocytose makropinosom endokytose pH V-ATPase protonpumpe reaktive oksygenarter ROS NADPH oxidase fagocytt makrofag dendrittisk celle
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