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Labeling of Extracellular Vesicles for Monitoring Migration and Uptake in Cartilage Explants

Article DOI: 10.3791/62780-v • 07:58 min • October 4th, 2021
October 4th, 2021 •
1,2, 1,2, 1,2, 3, 1,2,4, 1,2,4, 1,2, 1,2
1Cell Therapy and Tissue Engineering Group, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands, 2Health Research Institute of the Balearic Islands (IdISBa), 3Cellomics Unit, Institut Universitari d'Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, 4Fundació Banc de Sang i Teixits de les Illes Balears (FBSTIB)

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résumé
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Here, we present a protocol to label platelet lysate-derived extracellular vesicles to monitor their migration and uptake in cartilage explants used as a model for osteoarthritis.

Tags

Labeling Extracellular Vesicles Monitoring Migration Uptake Cartilage Explants Confocal Microscopy EV Imaging Therapeutics In Vitro Experiments In Vivo Experiments Concentration Purification Step Centrifuge Re-suspend Diluent C Aliquots Treatment Nanoparticle Tracking Analysis
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